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Annual report : National Institutes of Health. Division of Research Services (Volume 1987) online

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-30-



PHS bO-iO (Re



OHO Ul 4-&1 b



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



zoi Rs 00081-01 vn



PERIOD COVERED

June 15, 1987 to May 15, 1988



TITLE OF PROJECT (80 ctiaraciers or less. Title must lit on one line between the borders.)

Control of Luteal Function and Induction of Ovulation and Conception in the Anestrus Bitch



PRINCIPAL INVESTIGATOR (List other prolessional personnel below the Principal Investigator ) (Name, title, laboratory, and institute atliliation)



PI: P. K. Chakraborty

Others: R. L. Killens

J. L. Brown



Head, Research Division
Obstetrics & Gynecology
Chief, Carnivore Unit
Research Associate
Obstetrics & Gynecology



USUHS

ACS/VRB/DRS
USUHS



COOPERATING UNITS (il any)

Carnivore Unit, ACS, VRB, DRS; Research Division, Department of Obstetrics and
Gynecology, USUHS.



LAB/BRANCH

Veterinary Resources Branch



SECTION

Animal Center Section



INSTITUTE AND LOCATION

DRS, NIH, Bethesda, Maryland 20892



TOTAL MAN-YEARS;
.6



PROFESSIONAL:



.4



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects
n (a1) Minors
D (a2) Interviews



D (b) Human tissues



(c) Neither



SUMMARY OF WORK (Use standard unreduced type Do not exceed the space provided )

This project is designed to study the effect of two treatment regimens using hormone
stimulants to induce ovulation and conception in bitches. The major areas of interest
are: 1) the induction of regression of the functional corpora lutea by hormone
administration; and 2) the induction of follicular development and ovulation followed by
breeding bitches pretreated with hormone to regress corpora lutea.



-32-



PHS 6040 (Rev 1/64)



GPO 81 4-9ie



BEIB RESEARCH PROJECTS (ZOl RS)



10001-19 Pharmacokinetics

10002-22 Implant Device Development

10015-12 Development of Everting (Toposcopic) Catheter
for Clinical Vascular Use

10034-10 Three-Dimensional Histological Reconstruction

10039-10 Biophysical Instrumentation and Methodology

10043-10 Fiber Optics Probes

10096-07 Light Scattering Method for Evaluation of Platelets

10097-07 Studies in Cardiovascular Dynamics

10098-07 Laser Instrumentation for Vitreous and
Cardiovascular Microsurgery

10099-07 Cochlear Mechanics and Hair Cell Transduction

10103-07 Triple-Laser Multi-Parameter Row Cytometry
System for Study of Tumor Cell Kinetics

10109-07 Adjunctive Heat Treatment of Cancer

1 1 1 2-07 Analysis of Microcirculatory Blood Row by
Laser Doppler Scattering

101 16-06 Modeling of Arterial Pulse Waves

10122-06 Microcomputer applications for the NIH
Bio-Technology Unit (Pilot Plant)

10146-05 Prosthetic Urethral Sphincter

10151-05 Nuclear Magnetic Resonance Imaging

10156-05 Differential Scanning Calorimeter

10157-04 Temperature Controlled Chamber for X-ray
Diffraction Specimens

10162-05 Wound HeaUng: Biology and Rheology

10163-05 Magnetoencephalographic Localization of Foci
of Neurologic Activity

10170-05 Biological Applications of a Computer-
Controlled Analytical Electron Microscope



R.L. Dedrick


1


J.W. Boretos


5


D.R. Shook


8


S.B. Leighton


11


M.S. Lewis


13


J.I. Peterson


15


R.F Bonner


18


R.S.Chadwick


20


R.F. Bonner


23


R.S.Chadwick


26


W. Schuette


28


R.L. Levin


30


R.F. Bonner


34


R.S.Chadwick


37


T.R. Clem


39


S.B. Leighton


41


D.I. Hoult


43


C.P. Mudd


45


C.P. Mud


47


T.L. Talbot


49


P.D. Smith


51


R.D. Leapman


55



TABLE OF CONTENTS (Cont'd)



10184-04 Physical Chemistry of Biological Macromolecules

10185-04 Interventional Catheter Development

10193-04 Development of Everting Toposcopic Catheter
for Clinical Gastroenterological Use

10194-04 Optimized Polymer Processing for Advanced
Catheter Development

10201-03 Automated Cell-Colony Scanner System

10204-03 Cell Handling Studies

10207-03 CHnical Engineering Program, Clinical
Center Patient Areas

10210-03 Electronic Patient Monitoring System

1021 1-03 IEEE-488 GPIB Personal Computer

Instrumentation Use Program Development

10212-03 Fluoroimmunoassay Apparatus

10213-03 Vitreous Fluorophotometry Analysis

10214-03 Photoradiation Therapy

10215-03 Measurement of Trace-Level Metals and
Complexes in Biological Milieux

10216-03 Enzyme Purification - Calmodulin

102 1 8-03 Analytical Electron Microscopy of Adrenal
Chromaffin Cells

10219-03 Quantitative Elemental Mapping of
Rapidly-Frozen Neural Tissue

10225-03 Processing of High Resolution Electro Micrographs

10227-02 In Vivo Evaluation of Pulsed Intra- Arterial Infusions

10230-02 Television Tracking of Beating Hean Cells In-vitro

10232-02 Hardware Expansion for Flow Cytometery

10233-02 Computer System for Analysis of Monkey VocaUzations

1 0234-02 Computer Aided Tracking of Myosin Coated
Beads on Actin Filaments

10236-02 Instrumentation for Various Electron Microscopes



M.S. Lewis


58


J.W. Boretos


62


D.R.Shook


64


D.R. Shook


66


S.R.Goldstein


68


S.B. Leigh ton


70


R. Corsey


72


H.E. Cascio


73


T.R. Clem


75


W.S. Friauf


77


P.M. Bungay


79


P.D. Smith


83


A.F. LeRoy


87


A.F. LeRoy


91


R.D. Leapman


93


R.D. Leapman


96


M. Unser


98


D.R. Shook


101


W. Schuette


103


C.C. Gibson


105


C.C. Gibson


107


C.C. Gibson


109


C.C. Gibson


111



TABLE OF CONTENTS (Cont'd)



10238-02 Automation of BEIB Information Processing Functions J.R. Ellis

10240-02 Clinical Measurement of Microvascular Blood in
Sickle Cell Patients

1 024 1 -02 Cisplatin Kinetics

10247-02 The Effect of Mitral Valve Replacement On Left



10248-02 Intra- arterial Optical Evaluation

10249-02 Kinetics of Folate Metabolism

10250-01 Insulin Substrate and Energy Metabolism of Lung
Cancer Patients

10251-01 In Vivo System For Studying Tooth Pulp Vitahty

10252-01 Physiological Monitoring System for Sepsis Models

10253-01 Software Development for Removal of Plural

Inelastic Scattering from Electron Energy Spectra

10254-01 Electron Microprobe Elemental Imaging and
Quantitation of Alzheimer's Associated Plaque

10255-01 A Temperature Controlled Microscope Chamber for
Intracellular Electrical Activity Studies

10256-01 Mechanical Prosthetic Heart Valve Tester

10257-0 1 Analysis of Propagation of Light in Turbid
Biological Tissues

10258-01 Photochemical Inactivation of Virus
and Bacteria in Blood

10259-01 Visual Target Tracking Abihty Assessment System

1 0260-0 1 Real Time High Performance Confocal Laser
Scanning Optical Microscope

10261-01 Elemental Mapping of Trypanosoma Cruzi by
Analytical Electron Microscopy

10262-01 Hemodynamic Models for Catheter Studies

10263-01 A System for Quality Control of Injected Activity
for Short-Lived Radionuclides

1 0264-0 1 Microfluorometer for On-Line Measurements
of Intracellular pH



113



E.C. Walker


116


P.F. Morrison


119


T.L. Talbot


122


P.D. Smith


125


P.F. Morrison


129


R. Corsey


132


E. Walker


133


E.Walker


134


C. Swyt


135


C. Swyt


138


C.P. Mudd


141


T.L. Talbot


143


R.F. Bonner


144


R.F. Bonner


146


T.R. Clem


148


S.R. Goldstein


150


R.D. Leapman


152


R.L Lutz


154


A. Markowitz


159


A. Markowitz


161



TABLE OF CONTENTS (Cont'd)



10265-01 Quantitative, On-Line Detection of Secreted ATP

1 0266-0 1 CGR Collaborative Project in Magnetic
Resonance Imaging

10267-01 A Low Noise Electrometer for an Automated OEC
Measurement System

10268-01 Electroporation Apparatus

1 0269-0 1 Physiologic S tudy of High Altitude S heipas
at Their Homes

10270-01 Computer-Assisted Analysis of M-mode
Echocardiograms



A. Markowitz


163


A.L. LeRoy


165


H.E. Cascio


167


W.S. Friauf


169


C.C. Gibson


171


M. Unser


173



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



ZQ1 RS innni-iQ rfi



PERIOD COVERED

October 1, 198 6 to September 30,



1 987



TITLE OF PROJECT (80 charactarz or less. Title must III on one line between the borders.)

Pharmacokinetics



PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute alfiliationj

R.L. Dedrick Chief, CHES BEIB DRS



Others :
F .Farris
F.King
R.J. Lutz
P.F. Morrison



Southeastern Coll. of Pharm. Sci .
North Carolina A&T Univ.

Chemical Engineer BEIB DRS
Physical Scientist BEIB DRS



COOPERATING UNITS (il any)

CPB, NCI (J.M. Collins); DR-CC (J.L. Doppman) ; NTP-NIEHS (H.B.
Matthews); SNB-NINCDS (E.H. Oldf ield) ; University of Maryland

(M Egnr-i n )



LAB/BRANCH

Pti nmpdi <-;=i 1 F.ngi nf^p-r i ng ?inH Tn <:;i- r-np ient ? t 1 On



SECTION

Chemi ra 1 — Engineering Section



INSTITUTE AND LOCATION



DR S, Nationa] — Institutes of Health, Bethesda, — MD 20892



TOTAL MAN-YEARS



. 5



PROFESSIONAL



. 5



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects
n (a1) Minors
D (a2) Interviews



D (b) Human tissues



(c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

Pharmacokinetic models are developed for the distribution and
disposition of drugs, environmental contaminants, and endogenous
metabolites in animals and humans. They provide a plausible set
of equations that can be used to extrapolate data from animals to
humans and thereby improve chemotherapy and risk assessment.
Consideration of regional drug delivery has continued with
emphasis on intra-arterial and intracavitary administration. This
has led to spatially-distributed descriptions of the processes.
On theoretical bases, depth of penetration of drugs into tissue
adjacent to cavities can vary considerably, depending on the
intratissue diffusivity, capillary permeability-area product and
rate of irreversible reaction with tissue. Drug streaming from
arterial catheters appears to be a frequent problem leading to
nonuniform distribution of drug in the infused tissue and
compromising studies of toxicity and therapeutic effect. Work is
in progress on the development of a physiological pharmacokinetic
model for methyl mercury and inorganic mercury in the rat. Work
is also in progress on the adaptation of a pharmacokinetic model
for cis-diamminedichloroplatinum (II) to other platinum-containing
complexes .



PHS CO-IO (Rev. 1/84)



GPO 01 4-010



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



7ni RSinnn?-ppRFi



PERIOD COVERED



October 1. IQfifi to .Sep 1-p mbpr .10, 1 qR7



TITLE OF PROJECT (80 characters or less. Title must lit on one line between the borders.)

Implant Device Developrnpnt



PRINCIPAL INVESTIGATOR (Ust Other prolessional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation)

John W. Boretos Physical Scientist BEIB DRS



John Doppman, M.D.
Edward Oldfield, M.D.
F.T. Hambrecht, M.D.
Richard Clark, M.D.



Radiologist
Neurosurgeon
Health Science Adm,
Heart Surgeon



CR, CC
NINCDS
NINCDS
NHLBI



COOPERATING UNITS (it any)

CR, CC, NIH; NS, NINCDS, NIH
FNP, NINCDS, NIH; S, NHLBI, NIH
RF . TR, DRS, NTH .



LAB/BRANCH

Ri nmedi ca 1 — F.ngineerinq and — Instrumentation



SECTION

C.hemica.] — Engineerinr



INSTITUTE AND LOCATION



DRS, NTH Bethesda, MP



20892



TOTAL MAN-YEARS:



1 .0



PROFESSIONAL:



-0^:7-



-Q^^



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects
D (a1) Minors
n (a2) Interviews



D (b) Human tissues IS (c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

The purpose of this project is to study the interaction between
biomaterials and the physiological environment and to determine
the suitability of specially-prepared biomaterials for use in
various contexts. Polymers, metals and ceramics are important for
use in catheters, heart-assist pumps, electrode insulation and
similar implant applications. Variations in these materials as
well as physically induced stress and environmentally accelerated
degradation can severely reduce their effectiveness for long-term
use as surgical devices. Previous studies undertaken by this
project have shown a relationship between the molecular chain
structure and resistance to hydrolysis. Recent evidence suggests
that physical forces such as stress induced during fabrication can
promote a form of stress corrosion. In vitro test data and SEM
photomicrographs of surgical explants of various polyurethane
classes show that premature failure is often the result of a
combination of forces acting on the polymer at stress risers.
Polymer systems and composites capable of time dissolution offer
significant advantages in the development of devices that allow
natural tissues to take over as healing



PHS 6040 (Rev. 1/84)



GPO ei4-Bia



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RSinnis-i?RFi



PERIOD COVERED



October 1. 198 6 to .Sep t.e mhpr 30, 1987



TITLE OF PROJECT (80 characters or less. Tille must lit on one line between the borders.)

Development of Everting (Toposcopic) Catheter for Clinical Vascular Use

PRINCIPAL INVESTIGATOR (Ust other prolessional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation)



PI:


D.R. Shook


J.L


Doppman


E.H.


Oldfield


D.L.


Miller


H.R.


Keisar



Biomedical Engineer

Chief

Chief (Acting)

Radiologist

Clinical Director



BEIB



DRS



DR


CC


SNB


NINCDS


DRS


CC


DIR


NHLBI



COOPERATING UNITS (i! any)

Diagnostic Radiology, CC (J.L. Doppman, D. Miller);
Surgical Neurology, NINCDS (E.H. Oldfield)



U\B/BRANCH

Ri nrnpHi ca 1 F.ngi nppr i ng and Tn c;-t- rnmpnt a t i on Rranrh



SECTION

Mpchanical Knqinpprinq .9prt i nn



INSTITUTE AND LOCATION



DRS, National Tn.^^titntp^ of Hpalth^ Rpj-hP^Ha, MT) ?0S9?.



TOTAL MAN-YEARS:



n 1



PROFESSIONAL:



, 1



CHECK APPROPRIATE BOX(ES)

K" (a) Human subjects D (b) Human tissues □ (c) Neither

D (a1) Minors
D (a2) Interviews



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

The everting catheter has been shown to be a reliable clinical
means to catheterize long, small diameter, and highly tortuous
blood vessels, inaccessible by previous techniques. An everting
element evaginates from the tip of a conventional catheter. This
extremely flexible polyurethane element has been fabricated in 3,
4 and '5 French sizes, mated with 5, 6 and 7 French catheters,
respectively, and is capable of eversion lengths in excess of 4
cm.

The catheter has been applied clinically for the local delivery of
drugs to brain tumors and embolizing agents to the liver. In the
former, treatment is provided by positioning the conventional
catheter in the internal carotid artery from a femoral entry,
everting the element through the carotid sinus (beyond the
ophthalmic artery to avoid retinal toxicity) and perfusing the
tumor through the middle and/or anterior cerebral



PHS 6040 (Rev. 1/84)



GPo et4-sta



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



11

PROJECT NUMBER

zni R.9 inn.q4-in rfi



PERIOD COVERED



October 1. 1Q86 t.n Spptpmbpr .10, 1 qR7



TITLE OF PROJECT (80 characters or less. Title nnust lit on one line between the borders.)

Three-Dimensional Hi stnl ogical RRcnn.qf rnrt- i nn



PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation)

S.B. Leighton Mechanical Engineer BEIB DRS
A.M. Kuzirian Neuroanatomist LB NINCDS



COOPERATING UNITS (if any)

LB NINCDS



LAB/BRANCH



Ri nmedi ca 1 F.ngi nepri ng and InstrLimentation



SECTION



MPChan 1 c a 1 F.ngi nf^^-ri ng .q^n-h -j op



INSTITUTE AND LOCATION



National Tnstitntes of Health, Bethesda, MP 20892



OTOEF



TOTAL MAN-YEARS:



_2_



PROFESSIONAL:



CHECK APPROPRIATE BOX(ES)

n (a) Human subjects D (b) Human tissues K (c) Neither

D (a1) Minors
D (a2) Interviews



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

A semi-automatic system for acquiring three-dimensional structural
information about histological material is being developed. The
system should be faster and more reliable than techniques that use
serial sections, although resolution may be limited. In brief, an
embedded tissue block will be fixed relative to a scanning
electron microscope imaging system, the surface of the block will
be imaged and the image stored, and successive slices will be
removed by a built-in microtome. Handling and registration of
thin sections will thus be eliminated. Human and computer pattern
recognition will transform the resulting set of images into a
three-dimensional reconstruction. Oxygen plasma etching has been
found to give sufficient topographic relief that the resolution of
the images is now limited by the SEM and not by the preparation
technique. The images of Hermissenda Crassicornis obtained by
this technique correlate well with TEM images of the same tissue.



PHS 6040 (Rev 1/84) gpo »14-»i«



13



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



7 01 R8 in n.?9-i n rf i



PERIOD COVERED



nr-1-nhpr 1, 1Qi=!(^ tn P.f^pt^mh(=^r- ?n, 1 Qfi7



TITLE OF PROJECT (80 characters or less. Title must tit on one line between the borders.)



nhy.s-i r.a 1 Tnst rumpntal- i on ;^nH MA-t-hoHr^i r^rjy

INVESTIGATOR (List other protessional personnel below the Principal Investigator.) (f^am.



ame, title, laboratory, and institute affiliation)



Marc S. Lewis



Research Chemist



BEIB DRS



COOPERATING UNITS (if any)



LAB/BBANCH ,.,„. . jt^ ^ ^ .

Biomedical Engineering and Instrumentation



Iicroanalysis



''Y^fe^r^aTional Institutes of Health, MD 208 92



TOTAL MAN-YEARS:



0.1



PROFESSIONAL:



0.1



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects
D (a1) Minors
D (a2) lnterviev\/s



D (b) Human tissues



(c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

The project is designed to develop new instrumentation and methods
(or to improve existing ones) for characterization of biological
macromolecules and for study of their interactions. Analytical
ultracentrifugation, the techniques ancillary to it, and methods
of data analysis using mathematical modeling appropriate for these
techniques are the major areas of interest.

Major emphasis in this project has been given to applications of
mathematical modeling to problems in ultracentrifugal analysis.
This principal of concern has been to extend the method of
implicit constraints to a wider range of studies of macromolecular
interactions and in particular analyzing combined homogeneous and
heterogeneous associations. MLAB software, operating on the DEC-
10 computer, has been used for the mathematical modeling. The
applications of these studies are described in the project report
10184-04, Physical Chemistry of Biological Macromolecules.

A feasibility study continues toward development of a low-cost
optical data acquisition system (based upon fluorescence scanning)
that would convert existing preparative ultracentrif uges into
analytical ultracentrif uges .



PHS 6040 (Rev. 1/84)



CPO ai4-sis



15



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



701 R.qinn4;^-inRFi



PERIOD COVERED



October 1, 1986 to September 30^ 1987



TITLE OF PROJECT (80 characters or less. Title musf lit on one Una between the borders.)

Fiber Optic Probes



PRINCIPAL INVESTIGATOR (Ust other prolessional personnel below the Principal Investigator.) (Name, title, laboratory, and institute alfiliation)



John I. Peterson
Einar Stefansson



Chemist



BEIB, DRS
EICB



COOPERATING UNITS (it any)



RVR



LAB/BRANCH



aJ Engineering and — Instrumentation



rhf^nri i-a 1 Rngi nf^f^T i ng — Section



INSTITUTE AND LOCATION



DPS, Nat i onal In s t i tutes of Hea l th, Bethesda, MB 20892



TOTAL MAN-YEARS:



PROFESSIONAL



CHECK APPROPRIATE BOX{ES)

D (a) Human subjects D (b) Human tissues

n (a1) Minors
D (a2) Interviews



(c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

A suitable device is needed for the direct measurement of oxygen
partial pressure (PO2) in blood and tissue for both clinical and
research applications. Methods currently available for measuring
p02 lack convenience, reliability, speed, and applicability to many
situations of interest. Efforts to develop electrical sensors
have not been successful. It is desirable to have a very small p02
sensor which can be inserted into a blood vessel or tissue with
little disturbance, that will provide instantaneous p02 monitoring
for either short or extended periods of time. A fiber-optic
sensor is ideal for this application, with the advantage, for
physiological use, of very small size and flexibility, safety, and
low cost. A p02 sensor has been developed, based upon the principle
of fluorescence quenching by oxygen. The feasibility of the
sensor and its satisfactory performance have been demonstrated in
in vitro and in vivo tests in preceding years. The development of
the sensor as a needle probe has been accomplished.



PHS 6040 (Rev. 1/84)



CPO Dl 4-S1S



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



7ni RSinnq6-07REi



PERIOD COVERED



Ontn-hf^r- 1^ IQfif^ tn <S.p.pt ^my^^r -^H , 1QR7



TITLE OF PROJECT (80 characters or less. Title must lit on one Una between the borders.)

Li ght — Sraj-fpring Method -For- p-'ir?! 1 na-h i rM-i o-F p i at-^l f^-i- g



PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation)

BEIB, DRS

BEIB, DRS

BEIB, DRS

DBBP, CDB, FDA

BBPB, DBBP, CDB, FDA

Blood Bank, U.V.A

Blood Bank, U.V.A



R


F . Bonner


Physicist


T


R. Clem


Elect. Engineer


S


B. Leighton


Mech . Engineer


J


Fratatoni


Section Chief


B


Poindexter


Biologist


P


Mintz


Director


G


Anderson


Researcher



COOPERATING UNITS (it any)

Center for Drugs and Biologies, FDA
Blood Bank, U. Virginia



LAB/BBAMCH ,.,„. . j-r.. ^ ^ •

Biomedical Engineering and Instrumentation Branch



Electrical and Electronic Engineering



'Y3'&V^,^°^*ational Institutes of Health, Bethesda, MD 20892



TOTAL MAN-YEARS;



0.4



PROFESSIONAL:



0.4



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects
n (a1) Minors
D (a2) Interviews



(b) Human tissues D (c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

In the preceding years of this project we have developed a unique
instrument to assess noninvasi vely the concentration and
morphology of transfusion platelets via a rapid, clinically
acceptable procedure. The basis of this measurement is low-angle
multiple scattering of light and the changes in this signal
associated with orientation of the subset of' the platelet
population that is discoid. The instrument, under microprocessor
control, calculates the concentration and percentage of platelets
that are discoid within a transfusion bag. This requires fewer
than three minutes. These measurements are being used in a
variety of research studies of the effects of various storage
conditions (e.g., temperature transients, bag wall material,
addition of pharmacological agents) on the condition of stored
platelet concentrates. The instrument was originally designed to
play a role in improving the quality of platelet transfusions, by
routine and accurate noninvasive assessment of platelet quality
(as represented by the degree of normal platelet morphology) . To
this purpose ongoing studies at the Univ. of VA Medical School
Blood Bank are directed at determining the degree of correlation
between the noninvasive measurement and clinical efficacy of the
platelet transfusion.



PHS 6040 (Rev. 1/84)



GPO BI4-SIB



20



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT


2 4 5 6 7 8

Online LibraryNational Institutes of Health (U.S.) Division of RAnnual report : National Institutes of Health. Division of Research Services (Volume 1987) → online text (page 2 of 8)