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National Institutes of Health (U.S.) Division of R.

Annual report : National Institutes of Health. Division of Research Services (Volume 1987) online

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NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



2:01 RS 10210-03 BE!



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (SO characters or less. Title must lit on one line between the borders.)

Electronic Patient Monitoring System



PRINCIPAL INVESTIGATOR (List other prolessional personnel below the Principal Investigator.) (Name, title, laboratory, and institute atlitiation)

H.E. Cascio, Electronic Engineer, BEIB, DRS

Others :

G. Hemphill, Engineering Technician, BEIB, DRS

K. Musallam, Head Nurse, NURS, CC

D. Cirelli, Administrator Officer, OD, CC



COOPERATING UNITS (il any)
CCOD



°'^£'6Wr^dical Engineering and Instrumentation



-"S^P^ctrical and Electronic Engineering Section



gsg/ji^EAf^^^/^i^^^ Institutes of Health, Bethesda, MD 20892



TOTAL MAN-YEARS: Q _ g



PROFESSIONAL:



0.3



0.5



CHECK APPROPRIATE BOX(ES)

S (a) Human subjects D (b) Human tissues D (c) Neither

D (a1) Minors
D (a2) Interviews



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

An electronic patient monitoring system that alerts the nursing
staff when a patient (or patients) is leaving the nursing unit is
a welcome aid in the care of Alzheimer's disease patients and
other patients with dementia. The system design is based on a
wrist watch size radio transmitter, worn by the patient. A
detector unit, placed at the two doors, senses the presence of
any patient wearing a transmitter watch. With the detection of a
patient's transmitter, a microprocessor based controller locks
the exiting door, activates an alarm, and displays the patient's
name and location on a computer monitor screen. The personal
computer records the time and date for each patient's attempt to
leave the unit. This data may then be used for statistical
analysis. The number of patient monitoring channels has been
increased from four to eight. The system has been in continuous
operation and has become a dependable aid to the nursing staff.



PHS 6040 (Rev. 1/84)



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75



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10211-0.? RFI



PERIOD COVERED

October 1, 1986 to September 30. 1987



TITLE OF PROJECT (80 characters or less. Title must lit on one line between the borders.)

IEEE-488 GPIB Personal Computer Instnjmentation Use Program Development



PRINCIPAL INVESTIGATOR (List other prolessional personnel below the Principal Investigator.) (Name, title, laboratory, and institute atliliation)



T.R. Clem, Sr., Electronic Engineer, EEES, BEIB, DRS



COOPERATING UNITS (il any)



LAB/BRANCH

Biomedical Engineering and Instrumentation Branch



SECTION

Electrical and Electronic Engineering Section



INSTITUTE AND LOCATION

DRS. National In.qtitnte.s of Health, Rethesda, MP ?nfiq?



TOTAL MAN-YEARS:



PROFESSIONAL:



n.?.s



n.?



n ns



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects
D (a1) Minors
D (a2) Interviews



D (b) Human tissues



(c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

The increased availability and reduced cost of the small
Personal Computer has created increased interest in automating
data acquisition and process or experiment control in areas where
such things were not feasible before because of too high a cost
or great complexity. With these changes also came a .significant
increase' in the use of the IEEE-488 GPIB by instrument makers.
By combining the two, sophisticated instrumentation and data
acquisition systems can be assembled quickly and inexpensively.
The BEIB is continuing to develop the expertise to provide
guidance and assistance where this approach provides the optimal
solution. This capability is further assisted by the BEIB
Scientific Equipment Rental Program (SERP) specifying the IEEE-
488 interface on new equipment acquisitions whenever possible.
The increase at the NIH in the numbers and use of the IBM PC has
made this capability of great value to the NIH Intramural
Research program.



PHS 6040 (Rev. 1/84)



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77



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10212-03 BEI



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (80 characters or less. Title must lit on one line between the borders.)

Fluoroimmunoassay Apparatus



PRINCIPAL INVESTIGATOR (Ust other prolessional personnel belOM the Principal Investigator.) (Name, title, laboratory, and institute atliliation)

W.S. Friauf Ch . , EEES BEIB, DRS

R.L. Berger Physicist, LTD NHLBI

G. Hemphill Electronic Tech. BEIB, DRS



COOPERATING UNITS (il any)

CDC, Atlanta



U\B/BRANCH

Biomedical Engineering and Instrnmpnt-at i on Rrannh



Electrical and Electronic Engineering Section



INSTITUTE AND LOCATION

DRS. National Tn.qt i t-nfps of HP?i1t-h, R^i-h^=:d;=i , ^4^) ?0R9?



TOTAL MAN-YEARS:



0.2



PROFESSIONAL:

n. 1



, 1



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects D (b) Human tissues Kl (c) Neither

D (a1) Minors

D (a2) Interviews .



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

New rare earth chelate fluorescent probes with decay time
constants much longer than the background decay time-constant of
typical organic materials offer the potential of a great
improvement in sensitivity of time resolved fluorimetry.
However, if they are to rival radioimmunoassay methods the
sensitivity requirement becomes so great that overload recovery
of the fluorescence detector is a major problem. Extensive past
work on this problem is largely inapplicable to the very low
speed and level requirements of this situation. Consequently
initial effort is being applied to determining the optimum
fluorescence detection device and ancillary signal overload
limiting circuitry. Problems related to the fluorescent probe at
extremely low sample concentrations will be studied in
collaboration with CDC, which is interested in this approach as
another tool for AIDS research. Finally an evaluation of the
details of excitation, optical filtering, and digital signal
processing will be worked out and optimized.



PHS 6040 (Rev. 1/84)



CPO 014-818



79



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10213-03 BEI



PERIOD COVERED



October 1, 1986 to September 30, 1987



TITLE OF PROJECT (SO characters or less. Title must lit on one lirte between lt)e borders.)

Vitreous Fluorophotometry Analysis



PRINCIPAL INVESTIGATOR (List other prolessional personnel below the Principal Investigator.) (Name, title, laboratory, and institute aflilialion)

P.M. Bungay Chemical Engineer BEIB, DRS

R.F. Bonner Biophysicist BEIB, DRS

M.S. Roy Ophthalmologist CB, NEI

M.J. Podgor Statistician BE, NEI



COOPERATING UNITS (il any)

Clinical Branch, NEI; Off. of Biometry & Epidemiology, NEI



LAB/BRANCH ,,,„, . j-r^ ^ ^ ■ „

Biomedical Engineering and Instrumentation Branch



Chemical Engineering Section



■^£fe^,°^?fal:ional Institutes of Health, Bethesda, MD 20892



TOTAL MAN-YEARS: ^ ^



PROFESSIONAL:



0.5



CHECK APPROPRIATE BOX(ES)

Kl (a) Human subjects D (b) Human tissues D (c) Neither

D (a1) Minors
D (a2) Interviews



SU^4MAHY OF WORK (Use. standard unreduced type. Do not exceed Jfiesaace prqvided.) -, . .

Vitreous fluorophotometry (VFP) is a clinical procedure for
measuring the spatial distribution of fluorescence along the
optical axis in a human subject's vitreous following
administration of a fluorescent dye. In this project the

clinical instrument is a Fluorotron Master manufactured by
Coherent, Palo Alto, CA, and the dye is sodium fluorescein given
by intravenous injection. The purpose of the project is to
improve the protocols for conducting the measurements, processing
the output from the instrument, and analyzing the results from
the population of normal volunteers and patients.

One disease for which VFP may be useful is diabetes and the
associated pathology, diabetic retinopathy. The goals are to
evaluate the procedure for use in 1) screening for early
detection, and 2) monitoring for treatment efficiency. A

pharmacokinetic mathematical model has been developed- that
describes the rate of appearance of fluorescein in the vitreous
in terms of the blood-retinal permeability (characterized by a
permeability coefficient of the retina-vitreous interface) and
transport of fluorescein within the vitreous by diffusion
(characterized by a diffusion coefficient) . Plasma protein

binding of fluorescein is also incorporated in the model.



PHS 6040 (Rev. 1/84) cpo Bi4-aia



83



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10214-03 BEI



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (80 characters or less. Title must lit an one line between the borders.)

Photoradiation Therapy



PRINCIPAL INVESTIGATOR (Usi other prolessional personnel below the Principal Investigator.) (Name, title, laboratory, end institute affiliation)

Physicist BEIB DRS

Biophysicist BEIB DRS

Section Chief ROB NCI

Staff Fellow ROB NCI

Clin. Assoc. ROB NCI

Surgeon SURG NCI



PI:


P


D. Smith


Others :


R


F. Bonner




A.


Russo




M.


Manyak




T.


Delaney




H.


Pass




D.


Matthews



Veterinarian VRB



DRS



COOPERATING UNITS (if any)

ROB, NCI; VRB, DRS; SURG, NCI



LAB/BRANCH

Biomedical Engineering and Instrumentation Branch



SECTION

Electrical and Electronic Engineering Section



INSTITUTE AND LOCATION

DRS, National Institutes of Health, Bethesda, MD 20892



TOTAL WAN-YEARS:



2.5



PROFESSIONAL:



2.5



CHECK APPROPRIATE BOX(ES)

Kl (a) Human subjects
D (a1) Minors
D (a2) Interviews



D (b) Human tissues D (c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

Photodynamic therapy (PDT) , which is the interaction of light
with hematoporphyrin derivative (HDP, Photofrin II) , is in
clinical use in several areas of application. Currently, PDT is
being used clinically for a variety of skin lesions and for
bronchial obstructions and is being investigated in the
following: cancer of the prostate (beagles); carcinoma in situ
of the bladder and urinary tract (foxhounds); and pulmonary
metastatic disease (nude mice) . Specially designed fiber optic
probes and and introducers were manufactured for all cases, and
calibrated in terms of irradiance delivered to the tissue.
Photosensitized skin is a side effect of PDT, and the possibility
of using agents to protect against this photosensitivity was
addressed. In particular, hydrochlorothorazide appears to have a
pronounced effect on reducing skin photosensitivity in nude mice
models. Dosimetry for PDT is complex due to the variable
irradiation levels within the tissue, sequestering of HDP, and
tissue oxygenation. To address the first of these points and to



PHS 6040 (Rev. 1/84)



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87



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10215-03 BEI



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (80 characters or less. Title mt/sf til on one line befween the borders.)

Measurement of Trace-Level Metals and Complexes in Biological Milieux



PRINCIPAL INVESTIGATOR (Ust other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute etiiliation)



A.F. LeRoy, Ph.D.

M. Linnoila, M.D., Ph.

K. Flora, Ph.D.

M.K. Wolpert, Ph.D.

P. Galle,

B. Hecquet, Ph.D.

P. Parsons, Ph.D.

W. Thompson



BEIB, DRS

CPB, NIMH

PRB, NCI

Drug Eval. Br., DOT, NCI

Medical Faculty, Creteil, France

Centre Oscar Lambret, Lille France

BEIB, DRS

BEIB, DRS



COOPERATING UNITS (it any)

CPB-NIMH,DCT-NCI, PRB-NCI, Biophysics Dept . , Med. Faculty,
Creteil, France: Centre Anti-cancereux "Oscar Lambret", Lille,
France



LAB/BRANCH

Biomedical Engineering and Instrumentation Branch



SECTION

Analytical Methods



INSTITUTE AND LOCATION

DRS, National Institutes of Health, Bethesda, MP 20892



TOTAL MAN-YEARS:



1.20



PROFESSIONAL:



0.70



0.5



CHECK APPROPRIATE BOX(ES)

B (a) Human subjects
D (a1) Minors
D (a2) Interviews



D (b) Human tissues D (c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

Analytical methods have been developed for separation,
quantitation and characterization of elements (principally
metals) and their complex species (coordination compounds)
present in biological samples at trace levels. In vivo and in
vitro studies designed to produce biologically relevant results
require the ability to separate and quantitate the metals in the
low concentrations obtaining as well as the ability to relate
their concentrations to the biological milieux and their
components quantitatively.

To understand the mechanisms of action of the drugs under study
it is important to understand the interactions of the metal
complexes with proteins and other constituents. Unfortunately
many studies reported in the literature have been conducted at
much higher concentrations for want of analytical methods with
the requisite sensitivity. In the case of the platinum drugs
there is a further complicating factor in the relatively slow
kinetics of some of the reactions; many published studies assume
that the metal complex species and their interconversions are



PHS 6040 (Rev. 1/84)



91



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10216-03 BEI



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (80 characters or less. Title must tit on one line between the borders.)

Enzyme Purification - Calmodulin



PRINCIPAL INVESTIGATOR (Usi other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute atlilialion)

A.F. LeRoy, Ph.D. BEIB DRS

R. Berger, Ph.D. LTD NHLBI

W. Thompson BEIB DRS



COOPERATING UNITS (il any)



LAB/BRANCH

Biomedical Kngineering and Inst rnmpnf ?i1- i on



Analytical Methods



INSTITUTE AND LOCATION

DRS. National Institutes of HP^Ith, Rpfh^gH?^, Mn ?nfiQ?



TOTAL MAN-YEARS:



PROFESSIONAL:



0-1



n 1



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects
D (a1) Minors
D (a2) Interviews



D (b) Human tissues



(c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

The validity of studies of calmodulin's properties depends on
quantifiable measures of purity of the enzyme preparation and the
proportion of calcium it contains, and requires the availability
of extremely pure reagents, buffer, and solvents. Analytical
methods have been developed for quant itaiton • of picogram
quantities of calcium in highly purified preparations of
calmodulin and in the reagents and solvents needed to make the
preparations. Preliminary analyses show that the reagents and
solvents needed for making ultra-pure enzyme preparations can be
freed to satisfactory levels from such ubiquitous contaminants as
calcium using techniques developed in our laboratory.



PHS 6040 (Rev. 1/84)



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93



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10218-03 BEI



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (80 characters or less. Title must tit on one line between the borders.)

Analytical Electron Microscopy of Adrenal Chromaffin Cells

PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laborator/, and institute atliliation)



R.D. Leapman

Others :

R.L. Ornberg

H. Pollard



Visiting Scientist



Biophysicist
Chief



BEIB, DRS



LCBG, NIDDK
LCBG, NIDDK



COOPERATING UNITS (it any)



LAB/BRANCH

Biomedical Engineering and Instrumentation



SECTION

Electron Beam Imaging and Microspectroscopy Gr



onp



INSTITUTE AND LOCATION

DRS, National Institutes of Health, Rethe-:;da, MP POfiQ?



TOTAL MAN-YEARS:



PROFESSIONAL:



Q.8



n.R



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects
D (a1) Minors
n (a2) Interviews



D (b) Human tissues



(c) Neitirer



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

Elemental and water content of cultured bovine adrenal chromaffin
cells and their secretory granules have been measured and
compared with isolated chromaffin granules using rapid freezing,
ultracryomicrotomy, x-ray microanalysis and electron energy loss
spectroscopy. Changes in monovalent ion concentrations on
isolation were found to be large whereas calcium and phosphorus
concentrations remained approximately constant. Using measured
water contents of different compartments it was possible to
estimate a positive granule membrane potential (inside to
outside) of approximately 10 to 16 mV. Additional experiments
were performed to measure changes in granule composition by
adding ammonia to the culture medium to induce changes in
internal pH .

Cellular water content was determined using inelastic electron
scattering as measured by EELS. The method was first tested on
hydrated cryosections of standards and was then applied to
different compartments of the chromaffin cell.



PHS 6040 (Rev, 1/84)



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96



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10219-03 BEI



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (SO characters or less. Title must lit on ona line between the borders.)

Quantitative Elemental Analysis of Rapidly-frozen Neural Tissue



PRINCIPAL INVESTIGATOR (List other professional personnel belov/ the Principal Investigator.} (Name, title, lat>orator/, and institute atliliation)

R. D. Leapman Visiting Scientist BEIB

Others :

S.B. Andrews

T.S. Reese

D.M.D. Landis



Special Expert

Chief

Neurobiologist
Genetics and Anatomy, Case Western Reserve
University School of Medicine, Cleveland, Ohio



LN, NINCDS
LN, NINCDS
Dept .Developmental



COOPERATING UNITS (if any)



LAB/BRANCH

Biomedical Engineering and Instrumentation



SECTION

Electron Beam Imaging and Microspectroscopy Group



INSTITUTE AND LOCATION

DRS, National Institutes of Health, Bethesda, MP 20892



TOTAL MAN-YEARS:



0.2



PROFESSIONAL:



0.2



CHECK APPROPRIATE BOX(ES)

n (a) Human subjects
D (a1) Minors
D (a2) Interviews



D (b) Human tissues



(c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

Elemental analysis of incubated and freshly cut mouse cerebellar
slices was performed by means of energy-dispersive x-ray
spectroscopy in a Hitachi H700H analytical electron microscope.
Freeze-dried cryosections were probed in order to measure
potassium and calcium concentrations in Purkinje cell dendritic
spine organelles. The calcium content of spine cisterns from
"resting" synapses was found to be 2.5 ± 0.5 mmol/kg dry weight,
compared with a concentration of 6.7 ± .06 mmol/kg dry weight in
fresh (i.e., stimulated) synapses. The potassium concentration in
both the resting and stimulated synapses remained high and
approximately constant, indicating physiological integrity of the
tissue .



PHS 6040 (Rov. 1/84)



CPO ei 4-918



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10225-03 BE!



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (80 characters or less. Title must III on one line between the borders.)

Processing of High Resolution Electron Micrographs



PRINCIPAL INVESTIGATOR (List other prolessional personnel below the Principal Investigator.) (Name, title, laboratory, and institute etliliation)



Michael Unser
Benes Trus
Alasdair C. Steven



Visiting Fellow
Research Chemist
Visiting Scientist



BEIB, DRS
CSL, DCRT
LPB, NIADDK



COOPERATING UNITS (il any)



LAB/BRANCH

Biomedical Engineering and Instrumentation



SECTION

Office of the Chief



INSTITUTE AND LOCATION

DRS, National Institutes of Health, Bethesda, MP 20892



TOTAL MAN-YEARS;



1.0



PROFESSIONAL:



1.0



CHECK APPROPRIATE BOX(ES)

D (a) Human subjects
D (a1) Minors
D (a2) Interviews



D (b) Human tissues



(c) Neither



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

High resolution micrographs are often of poor quality, due to a
variety of distortions and especially due to a very low signal-to-
noise ratio. For micrographs of quasi-periodic arrays or sets of
images of ostensibly identical free-standing particles, visual
quality can be improved significantly by using correlation-
averaging techniques.

We have improved algorithms that we had previously developed for
the translational and rotational alignment of different views of
ostensibly identical specimens and for the compensation of spatial
deformations in quasi-periodic crystalline structures.

We have developed a technique for the quantitative assessment of
spatial resolution that is equally applicable to periodic and non-
periodic structures. This approach is based on the estimation of
a spectral signal-to-noise ratio.

These methods have been applied successfully to structure
determination and quantitative assessment of T7 Virus. They are
currently being used for the analysis of micrographs of skeletal
muscle filaments in both relaxed and rigor state.



PHS 6040 (Rev. 1/84)



cPO 01 4-oie



101



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10227-02 BEI



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (60 characters or less. Title must lit on one line between the borders.)

In Vivo Evaluation of Pulsed Intra-Arterial Infusions



PRINCIPAL INVESTIGATOR (Ust other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation)

D.R. Shook Biomedical Engineer BEIB DRS
S. Saris Staff Neurosurgeon SNB NINCDS



COOPERATING UNITS (if any)

Diagnostic Radiology, CC; Surgical Neurology, NINCDS;
Veterinary Resources Branch, DRS



LAB/BRANCH

Biomedical Engineering and Instrumentation Branch



SECTION

Mechanical Engineering Section



INSTITUTE AND LOCATION

DRS, National Institutes of Health, Bethesda, MD 20892



TOTAL MAN-YEARS:



0.02



PROFESSIONAL:



0.02



CHECK APPROPRIATE BOX(ES)

12 (a) Human subjects
D (a1) Minors
D (a2) Interviews



□ (b) Human tissues D (c) Neitiner



SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)

Diastolically-phased, pulsed intra-arterial infusions have been
shown to be an effective means to produce uniform mixing of blood
and drug, when measured in downstream branches of a model of the
cerebral circulation. The technigue has also been evaluated in
the cerebral circulation of fully anesthetized Rhesus monkeys by
infusion of radio-isotope-labelled iodoantipyrine into the
internal carotid artery. The resulting tissue concentration,
determined by quantification of autoradiographs, showed high
uniformity, indicating that the deleterious effects of infusate
streaming can be alleviated.



PHS 6040 (Rov. 1/84)



CPO o< 4-OI«



103



DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT



PROJECT NUMBER



Z01 RS 10230-02 BEI



PERIOD COVERED

October 1, 1986 to September 30, 1987



TITLE OF PROJECT (SO characters or less. Title must lit on one line between the borders.)

Television Tracking of Beating Heart Cells in-vitro



PRINCIPAL INVESTIGATOR (Ust other professional personnel beloM the Principal Investigator.) (Name, title, laboratory, and institute eftilialion)

W. Schuette Chief, ACES BEIB DRS

J. Parrillo Chief, CCMD CC

C. Burch CCMD CC



COOPERATING UNITS (il any)

DRS, BEIB
CCMD, CC



UkB/BRANCH

Biomedical Engineering and Instrumentation Branch



SECTION

Applied Clinical Engineering



INSTITUTE AND LOCATION

DRS, National Institutes of Health, Bfithesda, MP, 9089?


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